ICES Techniques in Marine Environmental Sciences
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Biological monitoring: General guidelines for quality assurance.
(International Council for the Exploration of the Sea (ICES), Copenhagen, Denmark, 2004)These guidelines have been prepared by the ICES/OSPAR Steering Group on Quality Assurance of Biological Measurements in the Northeast Atlantic (SGQAE), as part of its role to encourage the production of biological data of ... -
Biological effects of contaminants: Radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) techniques for the measurement of marine fish vitellogenins.
(International Council for the Exploration of the Sea (ICES), Copenhagen, Denmark, 2002)This document describes immunochemical methods to quantify the egg-yolk precursor protein vitellogenin in fish plasma. Vitellogenin is normally produced by the liver of mature female fish in response to 17β-oestradiol (E2) ... -
Chlorophyll a: Determination by spectroscopic methods.
(International Council for the Exploration of the Sea (ICES), Copenhagen, Denmark, 2001)Chlorophyll a is the principal pigment in plants. As a biomass indicator of aquatic microalgae which support food webs in the sea, it is probably the most frequently measured biochemical parameter in oceanography. This ... -
Biological effects of contaminants: Sediment bioassay using the polychaete Arenicola marina.
(International Council for the Exploration of the Sea (ICES), Copenhagen, Denmark, 2001)The method described here is a whole-sediment reworker bioassay using the polychaete Arenicola marina, a direct deposit feeder that is widely distributed in European coastal waters and on the east coast of North America. ... -
Biological effects of contaminants: Corophium sp. sediment bioassay and toxicity test.
(International Council for the Exploration of the Sea (ICES), Copenhagen, Denmark, 2001)The method described here is a whole-sediment reworker bioassay using burrowing amphipods. This method description covers the use of Corophium spp., as this is the genus most commonly used in Europe, but the procedure can ... -
Soft bottom macrofauna: Collection, treatment, and quality assurance of samples.
(International Council for the Exploration of the Sea (ICES), Copenhagen, Denmark, 1999)The aim of these recommendations is to standardize the methods used by different scientists for benthos surveys in order to increase the comparability of results for different areas. The results of ICES/HELCOM Quality ... -
Biological effects of contaminants: Measurement of DNA adducts in fish by 32P-postlabelling.
(International Council for the Exploration of the Sea (ICES), Copenhagen, Denmark, 1999)This document describes in detail the 32P-postlabelling method and its application to fish. Several recent studies have shown that the 32P-postlabelling method can be used to detect and measure the levels of DNA modified ... -
Biological effects of contaminants: Quantification of metallothionein (MT) in fish liver tissue.
(International Council for the Exploration of the Sea (ICES), Copenhagen, Denmark, 1999)This document describes methods to analyse the protein metallothionein in fish tissues. Metallothionein is induced by and binds essential (Cu, Zn) and non-essential (Cd, Hg) metals and is used in monitoring programmes as ... -
Biological effects of contaminants: Use of imposex in the dogwhelk (Nucella lapillus) as a bioindicator of tributyltin pollution.
(International Council for the Exploration of the Sea (ICES), Copenhagen, Denmark, 1999)This document describes a method for detecting contamination of the marine environment by tributyltin (TBT) using a sensitive neogastropod, the dogwhelk Nucella lapillus (L.), as a bioindicator. Exposure of female N. ... -
Biological effects of contaminants: Determination of CYP1A-dependent mono-oxygenase activity in dab by fluorimetric measurement of EROD activity.
(International Council for the Exploration of the Sea (ICES), Copenhagen, Denmark, 1998) -
Chlorobiphenyls in marine sediments: Guidelines for determination.
(International Council for the Exploration of the Sea (ICES), Copenhagen, Denmark, 1998)The analysis of chlorinated biphenyls in sediments generally includes extraction with organic solvents, clean-up, removal of sulphur, colullUl fractionation and gas chromatographic separation, mostly with electron capture ... -
Temporal trend monitoring: Robust method for analysing contaminant trend monitoring data.
(International Council for the Exploration of the Sea (ICES), Copenhagen, Denmark, 1998)This document describes a new method of assessing contaminant trends in fish muscle, fish liver, and shellfish. Previous methods of assessing trend data have often been complicated by the need to respond to unusual ... -
Control procedures: Good laboratory practice and quality assurance.
(International Council for the Exploration of the Sea (ICES), Copenhagen, Denmark, 1987)Good Laboratory Practice (GLP) is one of the manifestations of the increased attention being paid to quality control measures in general. It provides a framework designed to bring the quality ... -
Primary production: Guidlines for measurement by 14C incorporation.
(International Council for the Exploration of the Sea (ICES), Copenhagen, Denmark, 1987)In the years intervening between the preparation and publication of this do- cument, at least two other manuals for use in making primary production de- terminations have been introduced (Nie ... -
Lipophilic organic material: An apparatus for extracting solids used for their concentration from sea water.
(International Council for the Exploration of the Sea (ICES), Copenhagen, Denmark, 1987)Lipophilic organic substances, whether man-made, mobilized by human activities, or of recent natural origin, are usually dissolved in sea water at such minute concentrations that the chemical c ... -
Cadmium in marine sediments: Determination by graphite furnace atomic absorption spectroscopy.
(International Council for the Exploration of the Sea (ICES), Copenhagen, Denmark, 1987)Cadmium is one of the most important toxic elements to be determined in environmental samples. Cd has proved, however, to be a difficult element to determine with good precision and relative ... -
Protocol for measuring dioxin-like activity in environmental sample using in vitro reporter gene dr-luc assays.
(International Council for the Exploration of the Sea (ICES), Copenhagen, Denmark, 2013)Guidelines are given for the quantification of the dioxin-like activities of contaminants in sediment, biota and water samples using the DR-Luc reporter gene bioassay. Dioxins and dioxin-like compounds demonstrate high ... -
Oyster embryo-larval bioassay (revised).
(International Council for the Exploration of the Sea (ICES), Copenhagen, Denmark, 2013)The description of the oyster (Crassostrea gigas) embryo bioassay was initially published in the ICES TIMES series in 1991 (No.11). At the time, the bioassay was used in the United Kingdom for measuring water quality in ... -
Biological effects of contaminants: Paracentrotus lividus sea urchin embryo test with marine sediment elutriates.
(International Council for the Exploration of the Sea (ICES), Copenhagen, Denmark, 2012)This ICES Techniques in Marine Environmental Sciences describes a sediment elutriate bioassay using embryos of the sea urchin Paracentrotus lividus, a species widely distributed in both Atlantic and European Mediterranean ... -
Trace metals in sea water: Sampling and storage methods.
(International Council for the Exploration of the Sea (ICES), Copenhagen, Denmark, 1987)Sampling procedures for dissolved trace metals in sea water have progressed to the extent that it is now possible to describe reliable methods for the collection, preservation, and storage of ...